›› 2017, Vol. 38 ›› Issue (11): 3225-3230.doi: 10.16285/j.rsm.2017.11.018

• 基础理论与实验研究 • 上一篇    下一篇

微生物沉积碳酸钙固化砂土试验研究

孙潇昊1, 2,缪林昌1, 2,童天志1, 2,王呈呈1, 2   

  1. 1. 东南大学 交通学院,江苏 南京 210096;2. 东南大学 岩土工程研究所,江苏 南京 210096
  • 收稿日期:2016-12-05 出版日期:2017-11-10 发布日期:2018-06-05
  • 通讯作者: 缪林昌,男,1961年生,教授,博士生导师,主要隧道与地下工程研究。E-mail: Lc.miao@seu.edu.cn E-mail:sunxiao14hao@126.com
  • 作者简介:孙潇昊,男,1993年生,博士,主要从事隧道地铁的研究工作。
  • 基金资助:

    国家自然科学基金项目(No.51578147)。

Experimental study of solidifying sand using microbial-induced calcium carbonate precipitation

SUN Xiao-hao1, 2, MIAO Lin-chang1, 2, TONG Tian-zhi1, 2, WANG Cheng-cheng1, 2   

  1. 1. School of Transportation, Southeast University Nanjing, Jiangsu 210096, China; 2. Institute of Geotechnical Engineering, Southeast University, Nanjing, Jiangsu 210096, China
  • Received:2016-12-05 Online:2017-11-10 Published:2018-06-05
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (51578147).

摘要: 砂土固化对增加砂土强度、减小渗透性极为有利,可提高和改善砂土的力学特性。通过培养基L进行菌种繁殖,研究了不同条件下的细菌生长特性;分析流出液的pH值和Ca2+浓度的内在关系,揭示了固化砂柱的渗透性、无侧限抗压强度变化规律;通过微观结构阐释微生物固化效果,并研究了Ca2+浓度与试样强度的关系。结果表明:2 mL细菌母液加入100 mL培养液并在30 ℃、pH为6,150 rpm振动速度的条件下最适宜菌种生长;固化过程中,pH值逐渐降低,而Ca2+浓度则增大;采用0.5 mol/L胶凝液固化效果较好且固化周期短;固化后砂土颗粒间孔隙被生成的碳酸钙填充;试样渗透性最终降低3~4个数量级;流速越小,固化时间越长,固化效果越好;且固化试样破坏模式都是脆性破坏;采用尿素和醋酸钙混合溶液作为胶凝液,更能提高Ca2+利用率。

关键词: 砂土固化, 巴氏芽孢杆菌, 碳酸钙, 无侧限抗压强度, 微观结构

Abstract: Sand solidification is to increase the strength of sands and decrease infiltration. Culture medium was used to cultivate bacteria, and growth properties were obtained in various conditions. The relationship between pH and Ca2+ concentration of effluent was analyzed to reveal the evolution of permeability and unconfined compressive strength. Effect of solidification was investigated through microstructure. The relationship between Ca2+ concentration and strength of samples was studied. 2 mL of the bacterial mother liquor was added to 100 mL of the culture medium. The results show that the sporosarcina pasteurii possess optimal growth conditions under a pH of 6 and vibration velocity of 150 rpm with 30oC incubation. pH decreases during curing, and Ca2+ concentration increases. The 0.5 mol/L gel solution shows good curing effect and requires short time of the curing cycle. The gaps between sand particles are filled by calcium carbonate after curing, reducing sample permeability up to 3-4 order of magnitudes. The lower the rate of injection produces longer the curing time and the better the effect of solidification. The failure mode of all specimens is the brittle fracture mode. When the gelling solution is a mixture of urea and calcium acetate, the utilization ratio of Ca2+ is dramatically increased.

Key words: sand solidification, sporosarcina pasteurii, calcium carbonate, unconfined compressive strength, microstructure

中图分类号: 

  • TU 443

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