In order to study destruction law of aquiclude under the action of mining and seepage, this paper carries out the experiments of solid-liquid coupling similar simulation and the theoretical analysis of soil damage. The experimental results show that, in use of method of long wall mining in the interval mining, the combinatorial key stratum in shallow coal seam breaks into the fracture zone, and aquiclude breaks into the bending subsidence zone. The mining method can change the stratum moving destruction law and restrain the development of caving fissure into aquiclude, and avoid the connect with the bending subsidence zone. The method can achieve the purpose of protecting water-isolating capacity. Theoretical analysis of soil deformation and failure reveals that span of soil layer is span of the combinatorial key stratum breaking. The fractured water-conducting zone cannot be produced when the span doesn’t reach the limit deformation span in bending and sinking failure process. The mining practice has proved that water leakage doesn’t appear in the mining site after mining. The method is successfully used in a number of mining face exploitation to achieve water protection mining.