According to the engineering geological characteristics of bedding rock slope in Puli-Xuanwei Expressway in Yunnan province, the structural planes are divided into four types based on their formation mode. A home-made loading device is used to test the compression strength and shear strength of structural planes under natural conditions; and strength parameter values are obtained by least square linear fitting method, structural surface shear strength parameter values are obtained. By contrasting the equivalent shear strength of Mohr-Coulomb method based on JRC-JCS, the results basically agree with each other; and the feasibility of the device is verified. Considering filling thickness, moisture content of filler and other factors based on different types of slope structural planes, the shear strength parameters is obtained through several shear tests. The results show that the values of shear strength parameters in saturated state are smaller than that in the state of nature, meanwhile c increases and ψ decreases gradually with the increase of filling thickness. The test results provide reference data for correct evaluation of the characteristics of structure planes and stability calculation of the rock bedding slope in the next stage. This research results provide a simple test method for the choice of strength parameters relative to structural planes in the test field.