The scientific assessment of impact of tunnel engineering factor on groundwater environment is a technological problem to tunnel builders. The study of tunnel engineering factors on groundwater resources loss and level change is theoretical basis of scientific assessment of groundwater environment. Based on 350 km/h high-speed railway double line tunnel of grade V surrounding rock, the authors analyze the relationship between groundwater loss and level change with time. The authors further study the impact of multiple engineering factors on groundwater environment. The factors mainly include groundwater buried depth and tunnel construction method and construction time and shotcrete thickness and shotcrete anti-penetrability performance and shotcrete cracking leakage and strata grouting reinforcement method and grouting anti-penetrability performance and grouting range and partition wall. Finally, the authors suggest technical measures to protect groundwater environment during both construction stage and operation stage.