Based on typical scour shape, the lateral bearing capacity tests are conducted on typical nine-pile group foundation to simulate different local scour effects by changing scour depth, and parallel contrast tests on single pile foundation with the same parameters are also carried out. The load-displacement behavior, distribution of bending moment along pile shaft, efficiency coefficient of pile group and load sharing ratio of piles are analyzed in detail. The results show that as the scour depth increases, the lateral bearing capacities of single pile foundation and nine-pile group foundation are both weakened, the lateral efficiency coefficient of nine-pile group foundation increases gradually, the cap constraint effect becomes much more notable, the maximum bending moment along pile shaft increases, the maximum moment point of pile shaft comes down to the pile bottom, and the load sharing ratio of the front piles increases, while load sharing ratios of the rear and middle piles decrease. The front corner pile is severely affected by the scour depth and lateral load, so effective measures should be adopted to enhance the front corner pile in design.