Preparation of microbial induction of calcium carbonate precipitation(MICP) cemented sand samples injected twice bacteria solution with different concentrations of nutrient salt treatment, triaxial tests and CaCO3 quantitative chemical tests are conducted on cemented sand to analyze the effect of nutrient concentration on the mechanical properties of cemented sand and the relationship between the calcium carbonate content and the strength indexes of calcium carbonate precipitation samples. The results show that: The strength of the sample increase gradually with the increase of nutrient concentration; and then decrease after reaching a certain peak value under the same reaction time and volume of nutrient solution. The deformation modulus increases with the increase of dry density of samples under the condition of good distribution of calcium carbonate crystals. The distribution of calcium carbonate crystals and calcium carbonate content affected the increase of MICP specimen strength. The strength of 0.5 M is the highest in the experiment, and the content of calcium carbonate, cohesion and internal friction angle are 6.03%, 46.9 kPa and 41.31° respectively.