To address the limitations of traditional microbially
induced calcium carbonate precipitation (MICP) technology, including reliance
on exogenous bacterial strains, high costs, and poor applicability to
fine-grained loess, this study introduces environmentally friendly calcium
lignosulfonate into the cement solution instead of the traditional calcium
source, and optimizes concentrations of nutrient yeast extract (YE), NH4Cl,
urea-calcium source through single-factor and response surface Box-Behnken
design experiments. By targeting the activation of indigenous urease-producing
microorganisms for loess solidification, we systematically investigate the
solidification mechanisms using bioactivity monitoring, unconfined compressive
strength tests, calcium carbonate quantification, scanning electron microscope (SEM),
X-ray diffraction (XRD) and high-throughput sequencing. The results show that:
the optimized nutrient concentrations are 1.2 g/L for YE, 125 mmol/L for NH4Cl
and 0.8 mol/L for urea-calcium source; the urease activity, pH value, and viable
bacterial count in the optimized group peak at 120 hours of reaction,
significantly promoting calcium carbonate deposition; compared to the control
group (untreated loess), the optimized group exhibits 131.42%, 194.32%, and
734.65% improvements in unconfined compressive strength, secant modulus, and
calcium carbonate content, respectively; needle/rod-shaped calcium carbonate
crystals formed during the reaction significantly enhance soil strength and
compactness through filling-bridging-cementation effects; the relative
abundance of Bacillales in the optimized group reaches 93%, with notable
changes in microbial community diversity and composition. These findings
provide a reference for the engineering application of targeted activation
technology in loess regions.