The
leaching solution readily migrates through high-permeability zones within the
aquitard at in-situ leaching (ISL) uranium mining sites, leading to the
diffusion of uranium and other contaminants into surrounding groundwater, which
has become a critical bottleneck restricting the development of in-situ
leaching uranium mining technology. Microbially induced calcium carbonate
precipitation (MICP) exhibits effective cementation and permeability reduction
in rock and soil. It can be used to seal high-permeability zones within the aquitard,
but its standalone application often results in unsatisfactory sealing
performance. Therefore, this study applies mesoporous silica (MS)
synergistically with MICP to grout permeable sandstone from uranium mining
areas and investigates its cementation and water-stopping performance. Through
single-factor experiments, the effects of reaction time, grouting cycles,
initial bacterial concentration (OD600, the optical density measured
at a wavelength of 600 nm), urea/Ca²⁺ molar ratio, and initial pH on the amount
of calcium carbonate precipitation and the seepage reduction rate were
analyzed. The sandstone before and after cementation was characterized using
X-ray diffraction (XRD), scanning electron microscopy with energy dispersive
spectroscopy (SEM-EDS), and Fourier transform infrared spectroscopy (FTIR), to
reveal the mechanisms of permeability reduction by MICP-MS. The results show that the addition of MS increased both the
calcium carbonate content and the seepage reduction rate of the cemented sandstone.
With an MS addition amount of 4%, an initial bacterial OD600 of 1.2, a urea/Ca²⁺ ratio of 1.5:1.0, and an
initial pH of 7, after five grouting cycles, the MICP-MS treatment achieved a
seepage reduction rate of 90.72%. Microscopic analysis revealed that MS is rich
in various functional groups and acts as nucleation sites adsorbing Ca²⁺, which
promotes the precipitation of calcium carbonate. Moreover, MS and calcium
carbonate can form an MS-CaCO3 composite cement that jointly fills
pores and reduces the permeability coefficient of sandstone, effectively
preventing the diffusion and migration of the leaching solution. These results
provide reliable technical support for the green and sustainable development of
in-situ leaching uranium mining.