Rock and Soil Mechanics ›› 2022, Vol. 43 ›› Issue (S2): 307-314.doi: 10.16285/j.rsm.2021.1206

• Fundamental Theroy and Experimental Research • Previous Articles     Next Articles

Isolation and culture of a native urease-producing bacterium and its application in the reinforcement of fractured rock mass

CHENG Lei1, XIAO Yao1, DENG Hua-feng1, XIONG Yu1, PENG Meng1, ZHI Yong-yan1, LI Wen-hua2   

  1. 1. Key Laboratory of Geological Hazards on Three Gorges Reservoir Area, Ministry of Education, China Three Gorges University, Yichang, Hubei 443002, China; 2. China Three Gorges Projects Development Co., Ltd., Chengdu, Sichuan 610096, China
  • Received:2021-08-03 Revised:2022-03-30 Online:2022-10-10 Published:2022-10-03
  • Supported by:
    This work was supported by the National Nature Science Foundation of China (U2034203) and the Open Research Fund of Key Laboratory of Construction and Safety of Water Engineering of the Ministry of Water Resources, China Institute of Water Resources and Hydropower Research (202001).

PDF (Chinese)

85

Abstract: Microbial-induced carbonate precipitation is a novel green reinforcement technology in the geotechnical engineering field. To study the reinforcement effect of urease-producing bacteria extracted from the engineering environment in situ, a highly effective urease-producing bacteria was isolated from typical bank slope soil on the Three Gorges Reservoir and identified as Bacillus cereus. To analyze the reinforcement effect, the optimum culture conditions of Bacillus cereus were first determined by orthogonal optimization, and then the solidification tests on a sand column and fractured rock mass were carried out using Bacillus cereus and Sporosarcina pasteurii. The results showed that: (1) Compared with Sporosarcina pasteurii, the porosity of the sand column reinforced by Bacillus cereus was filled more densely, the impermeability was better, and the calcium carbonate engenderment was higher, thus designating that Bacillus cereus had better mineralization function. (2) The permeability coefficient of fractured rock samples reinforced by Bacillus cereus decreased by 3 orders of magnitude, and the internal friction angle and cohesion increased by 48.09% and 85.10% respectively，denoting that Bacillus cereus can efficaciously ameliorate the permeability and shear resistance of fractured rock, and compared with Sporosarcina pasteurii, it has a better reinforcement effect. Relevant research conceptions and results can provide a good reference for the green reinforcement of engineering rock mass.

Key words: Bacillus cereus, optimum culture conditions, sand column, fracture rock mass, slope reinforcement

CLC Number: 

  • TU452
[1] YANG Si-meng, PENG Jie, WEN Zhi-li, LIU Zhi-ming, LENG Meng, XU Peng-xu, . Application of concentrated seawater as calcium source solution in sand reinforcement using MICP [J]. Rock and Soil Mechanics, 2021, 42(3): 746-754.
[2] ZHANG Yu-fang, YUAN Kun,. Experimental study and application of anchor performance of a new type anchor cable with double anchor [J]. , 2018, 39(S1): 461-468.
[3] MA Jiu-chen, WANG Chang-feng, ZHU Long-hu, LIU Xue-ling，. Effects of particle morphology characteristics on transportation behavior of micro-nano particles in coastal energy storage brackish aquifers [J]. , 2017, 38(8): 2270-2278.
[4] ZHANG Si-yuan, ZHANG Yu-jun. 3D finite element analyses for anisotropy of deformation and strength of a cave in a dual-pore-fracture rock masses [J]. , 2016, 37(12): 3583-3590.
[5] JIANG Jian-qing , CAO Guo-hui , LIU Re-qiang,. Field test on behaviours of marine soft soil foundation treated with plastic drainage plate and sand column combined with surcharge preloading [J]. , 2015, 36(S2): 551-558.
[6] WANG Jin-mei , ZHANG Ying-bin , ZHAO John X , . Calculation model and algorithm for designing single anti-slide micropile [J]. , 2015, 36(8): 2395-2401.
[7] QIN Qian-bo ，FANG Yin-qing ，LUO Xing-wen ，HUANG Nian-hui. Test and analysis of effect of applying deep high pressure grouting method to stabilizing sliding zone of ancient debris landslide [J]. , 2012, 33(4): 1185-1190.
[8] WANG Huan-ling XU Wei-ya TONG Fu-guo . Coupled analysis of fracture rock mass slope saturated-unsaturated seepage field and stress field in flood discharge atomized rain area [J]. , 2008, 29(9): 2397-2403.
[9] ZHUANG Yan-feng , WANG Zhao , CHEN Lun . Model test of slope reinforcement through electro-osmosis and its numerical simulation based on energy analysis method [J]. , 2008, 29(9): 2409-2414.
[10] WANG Huan-ling , HE Miao , XU Wei-ya,. Research on porous medium hydraulic equivalence of fracture rock mass [J]. , 2007, 28(S1): 187-191.
[11] CHEN Wei-bing , ZHENG Ying-ren , LEI Wen-jie,. Three dimensional FEM simulation of slope reinforcement by deeply-buried pile [J]. , 2007, 28(S1): 273-276.
[12] ZENG Xiang-yong, DENG An-fu. Finite element analysis of rock cutting slope reinforced by combined action of prestressed anchor cable and anchor bolt [J]. , 2007, 28(4): 790-794.
[13] JIANG An-nan , LIANG Bing,. Forecasting grouting in fractured rock mass of underground engineering based on genetic algorithm-support vector machine [J]. , 2006, 27(S2): 141-145.
[14] WANG Huan-ling , XU Wei-ya ，TONG Fu-guo，. Study on slope saturated-unsaturated unsteady seepage under flood discharge atomized rain [J]. , 2006, 27(S2): 331-336.
[15] CHU Wei-jiang，XU Wei-ya，SU Jing-bo，REN Qiang，SHI Chong. Study of numerical model for large tunnel during construction process in water filling jointed rock mass [J]. , 2006, 27(S1): 156-160.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] CHEN Xu ，YU Jin ，LI Hong ，CAI Yan-yan ，ZHANG Ya-zhou ，MU Kang . Experimental study of propagation characteristics of acoustic wave in rocks with different lithologies and water contents[J]. , 2013, 34(9): 2527 -2533 .
[2] SUN Shao-rui ，WU Ji-min ，DONG Zhi-gao, WANG Wen-yuan, ZHOU Hong, WANG Kun， WEI Ji-hong，. Stability analysis of left bank B2 debris Jinanqiao Hydropower Station at Jinsha River [J]. , 2006, 27(S2): 407 -412 .
[3] ZHOU Hui , XU Rong-chao , LU Jing-jing , ZHANG Qi-feng , ZHANG Chuan-qing , MENG Fan-zhen,. Experimental study of instability destruction and crack propagation characteristics of slab failure model specimen[J]. , 2015, 36(S2): 1 -11 .
[4] LE Hui-lin, SUN Shao-rui. Effect of grouting materials and inclination angle of pre-existing flaw on uniaxial compressive strength and failure mode of rock-like specimens[J]. , 2018, 39(S1): 211 -219 .
[5] ZHI Yong-yan, DENG Hua-feng, XIAO Yao, DUAN Ling-ling, CAI Jia, LI Jian-lin. Analysis of seepage characteristics of fractured rock mass reinforced by microbial grouting[J]. Rock and Soil Mechanics, 2019, 40(S1): 237 -244 .
[6] XUE Yang, WU Yi-ping, MIAO Fa-sheng, LI Lin-wei, LIAO Kang, ZHANG Long-fei. Seepage and deformation analysis of Baishuihe landslide considering spatial variability of saturated hydraulic conductivity under reservoir water level fluctuation[J]. Rock and Soil Mechanics, 2020, 41(5): 1709 -1720 .
[7] FAN Jie, ZHU Xing, HU Ju-wei, TANG Yao, HE Chun-lei, . Experimental study on crack propagation and damage monitoring of sandstone using three-dimensional digital image correlation technology[J]. Rock and Soil Mechanics, 2022, 43(4): 1009 -1019 .
[8] TANG Xu-hai, XU Jing-jing, ZHANG Yi-heng, HE Qi, WANG Zheng-zhi, ZHANG Guo-ping, LIU Quan-sheng, . Determining mechanical parameters of asteroid rocks using NWA13618 meteorites and microscopic rock mechanics experiment[J]. Rock and Soil Mechanics, 2022, 43(5): 1157 -1163 .
[9] CHEN Rui, ZHANG Xing, HAO Ruo-yu, BAO Wei-xing. Shear strength deterioration of geopolymer stabilized loess under wet-dry cycles: mechanisms and prediction model[J]. Rock and Soil Mechanics, 2022, 43(5): 1164 -1174 .
[10] LIU Xin-xi, LI Bin, WANG Wei-wei, HE Cheng, LI Song. Calculation of active earth pressure of finite soil based on layered principal stress trajectory[J]. Rock and Soil Mechanics, 2022, 43(5): 1175 -1186 .
Copyright © Rock and Soil Mechanics, All Rights Reserved.
Tel: 027-87198484, 87199252, 87199253, 87198752　E-mail: ytlx@whrsm.ac.cn
Powered by Beijing Magtech Co. Ltd